The wq_bowtie program aligns sequences of genomes listed in a fastq file using Bowtie. It aligns by partitioning the file into pieces containing one or more sequences and running Bowtie to align them individually. The program uses the Work Queue framework for distributed execution. To run: 1. Install CCTools. 2. Install Bowtie and all its required dependencies. Bowtie can be download from: http://bowtie-bio.sourceforge.net/index.shtml 3. Run './wq_bowtie ' to align sequences in . 4. Start workers: work_queue_worker -d all where is the name of the host on which the master is running is the port number on which the master is listening. Alternatively, you can also specify a project name for the master and use that to start workers: 1. ./wq_bowtie -N WQBOWTIE 2. work_queue_worker -d all -N WQBOWTIE For listing the command-line options, do: ./wq_bowtie -h When the alignment completes, you will find the output files in the same directory from where wq_bowtie was run.